Beneficial Effect of Mannan Oligosaccharide on Caprine Hepatocyte Functional Parameters in vitro under Hyperthermia

Liver is involved in several important physiological functions of the body. In the present study, effect of hyperthermia and effect of supplementation of yeast cell wall component mannan oligosaccharide (MOS) under hyperthermic conditions on some of the functions of hepatocytes has been evaluated. Hepatocytes were isolated by disaggregation of liver piece with collagenase solution. It was observed that caprine hepatocytes were under thermal stress at 42 C as obtained with results on activity of LDH and antioxidant enzymes. The Least Square mean (LSM) values of the parameters were significantly higher when compared with the values estimated for hepatocyte culture at 37 C which served as control. Whereas the concentration of albumin decreased and TGF increased at 42 C when compared with estimated values for 37 C. On supplementation of MOS to hepatocyte culture at 42 C the results were reversed (P<0.01) by 72 h indicating protective effect of MOS on hepatocyte function. The results reported are based on in vitro studies only and further suggests that supplementation of MOS in feed to caprines under hyperthermic conditions may have beneficial effect on liver function. KEYWORDS